Independent Laboratory

 

  1. WANG B, ZHENG L, MERILLAT SA, FISCHER S, OCHABA J, THOMPSON LM, BARMADA SJ, SCAGLIONE KM, PAULSON HL. THE UBIQUITIN CONJUGATING ENZYME UBE2W REGULATES SOLUBILITY OF THE HUNTINGTON'S DISEASE PROTEIN, HUNTINGTIN. NEUROBIOL DIS. 2018 JAN; 109(PT A):127-136. PMCID:28986324

  2. SUTTON JR, BLOUNT JR, LIBOHOVA K, TSOU WL, JOSHI GS, PAULSON HL, COSTA MDC, SCAGLIONE KM, TODI SV. INTERACTION OF THE POLYGLUTAMINE PROTEIN ATAXIN-3 WITH RAD23 REGULATES TOXICITY IN DROSOPHILA MODELS OF SPINOCEREBELLAR ATAXIA TYPE 3. HUM MOL GENET. 2017 APR 15;26(8):1419-1431 PMID: 28158474

  3. Ristic G, Tsou WL, Guzi E, Kanack AJ, Scaglione KM, Todi SV USP5 is dispensable for mono-ubiquitin maintenance in Drosophila. J Biol Chem. 2016 Feb 25

  4. Santarriaga S, Petersen A, Ndukwe K, Brandt A, Gerges N, Bruns Scaglione J, Scaglione KM The Social Amoeba Dictyostelium discoideum Is Highly Resistant to Polyglutamine Aggregation. J Biol Chem. 2015 Oct 16;290(42):25571-8 PMCID: PMC4646202

  5. Zhang H, Amick J, Chakravarti R, Santarriaga S, Schlanger S, McGlone C, Dare M, Nix JC, Scaglione KM, Stuehr DJ, Misra S, Page RC A bipartite interaction between Hsp70 and CHIP regulates ubiquitination of chaperoned client proteins. Structure. 2015 Mar 3;23(3):472-82 PMCID: PMC4351142

  6. Blount JR, Tsou WL, Ristic G, Burr AA, Ouyang M, Galante H, Scaglione KM, Todi SV Ubiquitin-binding site 2 of ataxin-3 prevents its proteasomal degradation by interacting with Rad23. Nat Commun. 2014;5:4638 PMCID: PMC4237202

  7. Tsou WL, Burr AA, Ouyang M, Blount JR, Scaglione KM, Todi SV Ubiquitination regulates the neuroprotective function of the deubiquitinase ataxin-3 in vivo. J Biol Chem. 2013 Nov 29;288(48):34460-9 PMCID: PMC3843061

Postdoctoral Research

  1. Wang B, Merillat SA, Vincent M, Huber AK, Basrur V, Mangelberger D, Zeng L, Elenitoba-Johnson K, Miller RA, Irani DN, Dlugosz AA, Schnell S, Scaglione KM, Paulson HL Loss of the Ubiquitin-conjugating Enzyme UBE2W Results in Susceptibility to Early Postnatal Lethality and Defects in Skin, Immune, and Male Reproductive Systems. J Biol Chem. 2016 Feb 5;291(6):3030-42 PMCID: PMC4742764

  2. Faggiano S, Menon RP, Kelly GP, Todi SV, Scaglione KM, Konarev PV, Svergun DI, Paulson HL, Pastore A Allosteric regulation of deubiquitylase activity through ubiquitination. Front Mol Biosci. 2015;2:2 PMCID: PMC4428445

  3. Vittal V, Shi L, Wenzel DM, Scaglione KM, Duncan ED, Basrur V, Elenitoba-Johnson KS, Baker D, Paulson HL, Brzovic PS, Klevit RE Intrinsic disorder drives N-terminal ubiquitination by Ube2w. Nat Chem Biol. 2015 Jan;11(1):83-9 PMCID: PMC4270946

  4. Faggiano S, Menon RP, Kelly GP, McCormick J, Todi SV, Scaglione KM, Paulson HL, Pastore A Enzymatic production of mono-ubiquitinated proteins for structural studies: The example of the Josephin domain of ataxin-3. FEBS Open Bio. 2013;3:453-8 PMCID: PMC3829987

  5. Blair LJ, Nordhues BA, Hill SE, Scaglione KM, O'Leary JC 3rd, Fontaine SN, Breydo L, Zhang B, Li P, Wang L, Cotman C, Paulson HL, Muschol M, Uversky VN, Klengel T, Binder EB, Kayed R, Golde TE, Berchtold N, Dickey CA Accelerated neurodegeneration through chaperone-mediated oligomerization of tau. J Clin Invest. 2013 Oct;123(10):4158-69 PMCID: PMC3784538

  6. Smith MC, Scaglione KM, Assimon VA, Patury S, Thompson AD, Dickey CA, Southworth DR, Paulson HL, Gestwicki JE, Zuiderweg ER The E3 ubiquitin ligase CHIP and the molecular chaperone Hsc70 form a dynamic, tethered complex. Biochemistry. 2013 Aug 13;52(32):5354-64 PMCID: PMC3856692

  7. Scaglione KM, Basrur V, Ashraf NS, Konen JR, Elenitoba-Johnson KS, Todi SV, Paulson HL The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates. J Biol Chem. 2013 Jun 28;288(26):18784-8 PMCID: PMC3696654

  8. Todd PK, Oh SY, Krans A, He F, Sellier C, Frazer M, Renoux AJ, Chen KC, Scaglione KM, Basrur V, Elenitoba-Johnson K, Vonsattel JP, Louis ED, Sutton MA, Taylor JP, Mills RE, Charlet-Berguerand N, Paulson HL CGG repeat-associated translation mediates neurodegeneration in fragile X tremor ataxia syndrome. Neuron. 2013 May 8;78(3):440-55 PMCID: PMC3831531

  9. Thompson AD, Scaglione KM, Prensner J, Gillies AT, Chinnaiyan A, Paulson HL, Jinwal UK, Dickey CA, Gestwicki JE Analysis of the tau-associated proteome reveals that exchange of Hsp70 for Hsp90 is involved in tau degradation. ACS Chem Biol. 2012 Oct 19;7(10):1677-86 PMCID: PMC3477299

  10. Scaglione KM, Zavodszky E, Todi SV, Patury S, Xu P, Rodríguez-Lebrón E, Fischer S, Konen J, Djarmati A, Peng J, Gestwicki JE, Paulson HL Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. Mol Cell. 2011 Aug 19;43(4):599-612 PMCID: PMC3166620

  11. Todi SV, Scaglione KM, Blount JR, Basrur V, Conlon KP, Pastore A, Elenitoba-Johnson K, Paulson HL Activity and cellular functions of the deubiquitinating enzyme and polyglutamine disease protein ataxin-3 are regulated by ubiquitination at lysine 117. J Biol Chem. 2010 Dec 10;285(50):39303-13 PMCID: PMC2998082

  12. Todi SV, Winborn BJ, Scaglione KM, Blount JR, Travis SM, Paulson HL Ubiquitination directly enhances activity of the deubiquitinating enzyme ataxin-3. EMBO J. 2009 Feb 18;28(4):372-82 PMCID: PMC2646149

  13. Winborn BJ, Travis SM, Todi SV, Scaglione KM, Xu P, Williams AJ, Cohen RE, Peng J, Paulson HL The deubiquitinating enzyme ataxin-3, a polyglutamine disease protein, edits Lys63 linkages in mixed linkage ubiquitin chains. J Biol Chem. 2008 Sep 26;283(39):26436-43 PMCID: PMC2546540

Graduate Research

  1. Lass A, Cocklin R, Scaglione KM, Skowyra M, Korolev S, Goebl M, Skowyra D The loop-less tmCdc34 E2 mutant defective in polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2. Cell Div. 2011;6:7 PMCID: PMC3080790

  2. Scaglione KM, Bansal PK, Deffenbaugh AE, Kiss A, Moore JM, Korolev S, Cocklin R, Goebl M, Kitagawa K, Skowyra D SCF E3-mediated autoubiquitination negatively regulates activity of Cdc34 E2 but plays a nonessential role in the catalytic cycle in vitro and in vivo. Mol Cell Biol. 2007 Aug;27(16):5860-70 PMCID: PMC1952114

  3. Kim HT, Kim KP, Lledias F, Kisselev AF, Scaglione KM, Skowyra D, Gygi SP, Goldberg AL Certain pairs of ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s) synthesize nondegradable forked ubiquitin chains containing all possible isopeptide linkages. J Biol Chem. 2007 Jun 15;282(24):17375-86

  4. Deffenbaugh AE, Scaglione KM, Zhang L, Moore JM, Buranda T, Sklar LA, Skowyra D Release of ubiquitin-charged Cdc34-S - Ub from the RING domain is essential for ubiquitination of the SCF(Cdc4)-bound substrate Sic1. Cell. 2003 Sep 5;114(5):611-22